Development of an immunochromatographic test to detect antibodies against recombinant Em18 for diagnosis of alveolar echinococcosis.
نویسندگان
چکیده
An immunochromatographic test (ICT) for the rapid detection of antibodies to Echinococcus multilocularis was developed. The ICT showed a sensitivity of 94% and a specificity of 95.4%. High degrees of agreement were observed between the ICT and an enzyme-linked immunosorbent assay (kappa = 0.93) and between the ICT and immunoblot analysis (kappa = 0.97). It is expected that the ICT developed in this study will be useful for the serodiagnosis of alveolar echinococcosis.
منابع مشابه
Comparison of the serological tests ICT and ELISA for the diagnosis of alveolar echinococcosis in France☆
Serological diagnosis of alveolar echinococcosis (AE) is a key element for efficient patient treatment management. A rapid immunochromatography test kit (ICT) using the recombinant Em18 antigen (rEm18) was recently developed. The aim of our study was to assess this test on a panel of sera from French patients with alveolar echinococcosis and control patients. In a blind test, a total of 112 ser...
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Alveolar echinococcosis (AE) is the most potentially lethal parasitic zoonosis of the nontropical areas in the northern hemisphere, where cystic echinococcosis (CE) is also endemic. Both AE and CE are highly endemic in China, and both serologic detection of echinococcosis, either AE or CE, and differentiation of AE from CE are crucial problems. Evaluation of Western blot analysis (WB) and enzym...
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Western blot analysis was carried out in order to evaluate new serodiagnostic markers, Em18 and Em16, for differentiation of alveolar echinococcosis (AE) from cystic echinococcosis (CE) using 36 serum samples from hydatid patients from Xinjiang, China, where AE and CE are both endemic and one double infection case has been reported. All AE cases except one (5/6) who exhibited a calcified lesion...
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The Echinococcus multilocularis protein Em18 is one of the most promising antigens for use in serodiagnosis of alveolar echinococcosis in human patients. Here we identify an antigenic relationship between Em18 and a 65-kDa immunodominant E. multilocularis surface protein previously identified as either EM10 or EmII/3. The NH(2)-terminal sequence of native Em18 was determined, revealing it to be...
متن کاملImmunological and Molecular Tools for Identification of Echinococcosis and for Epidemiological Studies and the Present Problems in Japan
Recent advances in immunological and molecular approaches for the better resolution of immunodiagnosis of echinococcosis, either in humans or in animals, are briefly overviewed since April 1999. National echinococcosis surveillance in Japan has been performed. However, the lack of an evaluation system to confirm echinococcosis, either alveolar or cystic forms, the echinococcosis cases reported ...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 47 1 شماره
صفحات -
تاریخ انتشار 2009